fa بررسی فراوانی ژن‌های انتروسینA، انتروسین P و انتروسین As-48 در ایزوله‌های انتروکوکوس فکالیس جدا شده ازگوشت مرغ در شهرستان شهرکرد پژوهشی اصیل Original Article <span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">باکتریوسین&shy;های تولید شده توسط باکتری&shy;ها خاصیت ضد میکروبی دارند و برای نگهداری غذا به کار می‌روند. در مطالعات‌ اخیر توجه ویژه‌ای به این باکتری‌ها جهت بهره‌گیری از آن‌ها به‌عنوان نگهدارنده‌های مواد غذایی معطوف شده است. در این مطالعه تعداد 100</span></span></span><span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> نمونه گوشت مرغ،</span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> پس از جداسازی باکتری و تشخیص قطعی در حضور پرایمرهای اختصاصی مربوط به ژن‌های انتروسین مورد بررسی قرار گرفتند</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ژن مربوط به <i>انتروسین </i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">A</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در 17 ایزوله (</span></span></span><span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">07/32 </span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">درصد)، ژن مربوط به <i>انتروسین </i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در </span></span></span><span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">16 </span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ایزوله (18/30</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">درصد)، ژن مربوط به <i>انتروسین </i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">As-48</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در 11 ایزوله </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">21</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">درص</span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">د) بود. </span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">وجود هم</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">‌زمان چند ژن با همدیگر در </span></span></span><span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">10</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ایزوله (86/18</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">درصد) مشاهده‌گردید به طوری که ژن مربوط به <i>انتروسین‌های </i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&nbsp;A</span></span></span></span></i><i><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span></i> <i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span> <span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">3 </span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ایزوله (43/9 درصد)،</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ژن مربوط به <i>انتروسین</i></span></span></span><i><span lang="FA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">‌</span></span></span></i><i><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">های</span></span></span></i> <i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">A</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">As48</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در 2 ایزوله </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">77/3 درصد)، ژن مربوط به <i>انتروسین‌های </i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">A</span></span></span></span></i><i><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">،</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> P</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">As48</span></span></span></span></i> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">در 3 ایزوله (66/5</span></span></span> <span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">درصد) مشاهده گردید. با توجه به حضور تعداد نسبتاً زیادی از ژن‌های انتروسین در ایزوله‌های <i>انتروکوکوس فکالیس</i> جداشده از گوشت مرغ، انجام تحقیقات جهت بررسی خواص ضد میکروبی انتروسین</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">‌</span></span></span></span><span lang="AR-SA" style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">های تولید شده توسط این باکتری ضروری به نظر می‌رسد.</span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bacteriocins produced by bacteria have antimicrobial properties and are used to preserve food. In recent studies, special attention has been paid to these bacteria in order to use them as food preservatives. In this study, 100 chicken meat samples were analyzed after bacterial isolation and definitive diagnosis in the presence of specific primers related to enterocin genes. The gene related to <i>enterocin A</i> was in 17 isolates (32.07%), the gene related <i>to enterocin P</i> was in 16 isolates (30.18%), the gene related to <i>enterocin As-48</i> was in 11 isolates (21%). The simultaneous presence of several genes with each other was observed in 10 isolates (18.86%), so that the gene related to <i>enterocins A</i> and <i>P</i> in 3 isolates (9.43%), <i>enterocins A</i> and <i>As48</i> in 2 isolates (3.77%). The gene related to <i>enterocins A, P</i> and <i>As48 </i>was observed in 3 isolates (5.66%). Considering the presence of a relatively large number of enterocin genes <i>in Enterococcus faecalis</i> isolates isolated from chicken meat, conducting research to investigate the antimicrobial properties of enterocins produced by this bacterium seems necessary.</span></span></span></span></span></span></span> گوشت مرغ , انتروکوکوس فکالیس, انتروسینA, انتروسین P , انتروسین As-48 chicken meat, Enterococcus faecalis, enterocin A, enterocin P, enterocin As-48 46 55 http://zoonosis.ir/browse.php?a_code=A-10-171-4&slc_lang=fa&sid=1 Fahimeh nourbakhsh فهیمه نوربخش nourbakhshf951@mums.ac.ir 1080154388 1003194753284600237 No Elaheh Tajbakhsh الهه تاجبخش ee_tajbakhsh@yahoo.com 4621573381 1003194753284600238 No Parisa Behshood پریسا بهشود Parisa_behshood@yahoo.com 1292052503 1003194753284600239 Yes