Volume 3, Issue 4 (12-2023)
Zoonosis 2023, 3(4): 0-0 |
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Ethics code: IR.IAU.SHK.REC.1400.034
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nezaratizade S, khamesipour F, Hejazi S H. In vitro study of the anti-leishmanial effect of the essential oil of Dracocephalum kotschyi compared to common drugs on the growth of amastigotes of Leishmania major parasite. Zoonosis 2023; 3 (4)
URL: http://zoonosis.ir/article-1-98-en.html
URL: http://zoonosis.ir/article-1-98-en.html
Food and Drug Research Center , faham.khamesipour@yahoo.com
Abstract: (22 Views)
Leishmaniasis, a zoonotic parasitic disease, is recognized as a major public health concern. Current therapeutic regimens based on pentavalent antimonial compounds are associated with significant limitations and adverse effects, highlighting the urgent need for effective alternative treatments. Medicinal plants represent promising sources for the development of novel therapeutic agents. The aim of the present study was to evaluate the antileishmanial activity of the essential oil of Dracocephalum kotschyi against the amastigote form of Leishmania major and to compare its efficacy with standard drugs. In this experimental in vitro study, promastigotes of the standard strain of L. major were cultured in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS). Subsequently, macrophages infected with the parasite amastigotes were exposed to different concentrations of D. kotschyi essential oil. Amphotericin B was used as the positive control. Following incubation, cytotoxicity and amastigote survival rates were assessed using the MTT colorimetric assay, and optical density (OD) values were measured by an ELISA reader. Finally, the 50% inhibitory concentration (IC50) was determined for the essential oil and the control drug. The findings demonstrated that D. kotschyi essential oil exhibited a dose-dependent inhibitory effect on the growth of intracellular amastigotes (p < 0.05). The IC50 of the essential oil against amastigotes was calculated as 1136.53 µg/mL, while the 50% cytotoxic concentration (CC50) on macrophage cells was 193.60 µg/mL. The essential oil of D. kotschyi showed considerable potential as an antileishmanial agent and effectively eliminated infected macrophages. Therefore, this plant-derived compound could serve as a promising candidate for further in vivo investigations aimed at developing topical formulations for the treatment of cutaneous leishmaniasis.
Book Review: Original Article |
Subject:
Medical Parasitology
Received: 2025/09/29 | Accepted: 2025/12/4
Received: 2025/09/29 | Accepted: 2025/12/4
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