Volume 2, Issue 1 (6-2022)                   Zoonosis 2022, 2(1): 37-45 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Behshood P, tajbakhsh E, Nourbakhsh F. Prevalence of enterocin genes in Enterococcus faecium strains isolated from red meat in Shahrekord, Iran. Zoonosis 2022; 2 (1) :37-45
URL: http://zoonosis.ir/article-1-38-en.html
, ee_tajbakhsh@yahoo.com
Abstract:   (573 Views)
Bacteriocins are proteins produced by bacteria, especially lactic acid bacteria, which have antimicrobial properties and are used to preserve foods. L50A and l50B are the compounds produced by some Enterococcus faeicum strains. The present study was conducted to isolate and determine the frequency of enterocin L50A and L50B genes in E. faecium isolates from meat in Shahrekord, Iran. In this research, 80 meat samples were prepared, and the prevalence of l50A and l50B enterocin genes were studied. The bacteria were isolated using biochemical microbiology methods such as the bile esculin test, growth in NaCl 6.5%, pyruvate fermentation, and molecular techniques. In the presence of specific primers for enterocin genes, the frequency of l50A and l50B genes was determined. In this research, out of a total of 80 studied samples, 66 samples (82.5%) were infected with Enterococcus, of which 35 samples (43.75%) were infected with E. faecium bacteria. L50A was determined in 15 isolates (42.85%), and L50B was determined in 14 isolates (40%). Due to the relatively high contamination of E. faecium in meat, other studies for antimicrobial properties of enterocrins produced by this bacterium are recommende
Full-Text [PDF 605 kb]   (253 Downloads)    
Book Review: Original Article | Subject: Food Safety and Hygiene
Received: 2022/06/30 | Accepted: 2022/07/19 | Published: 2022/06/20

References
1. Radmehr M, Khashei Varnamkhasti K, Tajbakhsh E. Accuracy of REP-PCR Method in Genotyping of Enterococcus faecium Isolated From Red Meat as a Cause of Foodborne Infections. Armaghane Danesh. 2021;26(1):104-16.
2. Dapkevicius MdLE, Sgardioli B, Câmara SP, Poeta P, Malcata FXJF. Current trends of enterococci in dairy products: A comprehensive review of their multiple roles. 2021;10(4):821. [DOI:10.3390/foods10040821] [PMID] [PMCID]
3. Yousefi L, Ehsani M, Fazeli M, Mojgani N, Ezatpanah H. Characterization of enterocin-like substances produced by two strains of Enterococci isolated from ewe's and goat's milks. Iranian Journal of Nutrition Sciences & Food Technology. 2011;6(1):33-42.
4. Mirhosseini M, Nahvi I, Emtiazi G, Tavasoli MJWASJ. Culture-dependent and culture-independent qualitative analysis of dairy products for bacteriocin production by lactic acid bacteria. World Applied Sciences Journal. 2008;5(1):20-24.
5. Ronconi MC, Merino LAJEIMC. Prevalencia de Enterococcus faecalis y Enterococcus faecium con resistencia de alto nivel a aminoglucósidos en las ciudades de Resistencia y Corrientes, República Argentina. Enfermedades Infecciosas y Microbiología Clínica. 2000;18(2):71-3.
6. Salehi M, Hatami Z. Molecular Occurrence of Enterocin A Gene among Enterococcus faecium Strains Isolated from Gastro-Intestinal Tract and Antimicrobial Effect of this Bacteriocin Against Clinical Pathogens. Iranian Journal of Medical Microbiology. 2014;8(1):18-26.
7. Murray BE. The life and times of Enterococcus. Clinical Microbiology Reviews. 1990;3:46-65. [DOI:10.1128/CMR.3.1.46] [PMID] [PMCID]
8. Barbosa J, Gibbs PA, Teixeira P. Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal. Food Control. 2010;21(5):651-6. [DOI:10.1016/j.foodcont.2009.10.002]
9. Cosentino S, Podda G S, Corda A, Fadda ME, Deplano M, Pisano MB. Molecular detection of virulence factors and antibiotic resistance pattern in clinical Enterococcus faecalis strains in Sardinia. Journal of preventive medicine and hygiene. 2010;51(1):31-36
10. Giraffa G. Enterococci from foods. FEMS Microbiology Reviews. 2002;26(2):163-71. [DOI:10.1111/j.1574-6976.2002.tb00608.x] [PMID]
11. De Vuyst L, Foulquié Moreno MR, Revets H. Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. International Journal of Food Microbiology. 2003;84(3):299-318. [DOI:10.1016/S0168-1605(02)00425-7]
12. Cleveland J, Montville TJ, Nes IF, Chikindas ML. Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology. 2001;71(1):1-20. [DOI:10.1016/S0168-1605(01)00560-8]
13. Pandey N, Malik RK, Kaushik JK, Singroha G. Gassericin A: a circular bacteriocin produced by lactic acid bacteria Lactobacillus gasseri. World journal of microbiology & biotechnology. 2013;29(11):1977-87. [DOI:10.1007/s11274-013-1368-3] [PMID]
14. Cetinkaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clinical Microbiology Reviews. 2000;13(4):686-707. [DOI:10.1128/CMR.13.4.686] [PMID] [PMCID]
15. Christopher LP, Kapatral V, Vaisvil B, Emel G, Deveaux LC. Draft Genome Sequence of a New Homofermentative, Lactic Acid-Producing Enterococcus faecalis Isolate, CBRD01. Genome Announcements. 2014;2(2):e00147-14. [DOI:10.1128/genomeA.00147-14] [PMID] [PMCID]
16. Hickey RM, Twomey DP, Ross RP, Hill CJM. Production of enterolysin A by a raw milk enterococcal isolate exhibiting multiple virulence factors. 2003;149(3):655-64. [DOI:10.1099/mic.0.25949-0] [PMID]
17. Barzam E, Tajbakhsh E, Rahimi E. Prevalence of entrocins produced by Entrococcus faecalis isolated from traditional dairy products in Shahrekord City. Journal of Food Microbiology. 2015;2(2):79-87.
18. Kubašová I, Diep DB, Ovchinnikov KV, Lauková A, & Strompfová V. Bacteriocin production and distribution of bacteriocin-encoding genes in enterococci from dogs. International journal of antimicrobial agents. 2020;55(2):105859. [DOI:10.1016/j.ijantimicag.2019.11.016] [PMID]
19. Messi P, Guerrieri E, de Niederhäusern S, Sabia C, Bondi M. Vancomycin-resistant enterococci (VRE) in meat and environmental samples. International Journal of Food Microbiology. 2006;107(2):218-22. [DOI:10.1016/j.ijfoodmicro.2005.08.026] [PMID]
20. Piard JC, Desmazeaud M. Inhibiting factors produced by lactic acid bacteria.2: bacteriocins andother antibacterial substances. Lait. 1991;72:113-42. [DOI:10.1051/lait:199229]
21. Özdemir GB, Oryaşın E, Bıyık HH, Özteber M, Bozdoğan B. Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian journal of microbiology. 2011;51(2):182-7. [DOI:10.1007/s12088-011-0143-0] [PMID] [PMCID]
22. Strompfová V, Lauková A, Simonová M, Marciňáková M. Occurrence of the structural enterocin A, P, B, L50B genes in enterococci of different origin. Veterinary Microbiology. 2008 10;132(3-4):293-301. [DOI:10.1016/j.vetmic.2008.05.001] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 All Rights Reserved | Journal of Zoonosis

Designed & Developed by : Yektaweb