Department of pathobiology, Islamic Azad University, Karaj branch, Karaj, Iran , ZARE396@YAHOO.COM
Abstract: (245 Views)
Background: Wolbachia bacteria, which have been identified in many insects including mosquitoes, are intracellular bacteria and can be transmitted vertically. Despite numerous studies on the prevalence of Wolbachia in Cimex lectularis population, no published studies have reported on this topic among tropical bedbug’s populations in Iran. The present study was conducted to investigate the molecular prevalence of Wolbachia bacteria among tropical bedbug from Mashhad city.
Material and method: Samples of tropical bed bugs were collected from 4 different areas of Mashhad city, including regions 2, 11, 4, and 8. The sampling locations included old apartments and workers' dormitories. In total, 100 bed bug specimens were collected from 4 different areas of Mashhad city. The molecular identification method of Wolbachia bacteria was performed based on the Wolbachia surface protein and comparison with other registered Wolbachia sequences in the genebank.
Results: According to the present study, 13 samples of C. hemipterus (13%) from 2 different colonies in regions 4 and 8 were reported to be positive. Based on the results of sequence determination of a fragment of the WSP gene, 100% homology with the sequence of this gene in the registered genebank of Wolbachia was reported.
Conclusion: The present study was the first epidemiological study in Khorasan Razavi. This study allowed us to investigate the prevalence of Wolbachia bacteria in C. hemipterus in different populations from various areas of Mashhad city and compare it with the results of other similar studies.
Material and method: Samples of tropical bed bugs were collected from 4 different areas of Mashhad city, including regions 2, 11, 4, and 8. The sampling locations included old apartments and workers' dormitories. In total, 100 bed bug specimens were collected from 4 different areas of Mashhad city. The molecular identification method of Wolbachia bacteria was performed based on the Wolbachia surface protein and comparison with other registered Wolbachia sequences in the genebank.
Results: According to the present study, 13 samples of C. hemipterus (13%) from 2 different colonies in regions 4 and 8 were reported to be positive. Based on the results of sequence determination of a fragment of the WSP gene, 100% homology with the sequence of this gene in the registered genebank of Wolbachia was reported.
Conclusion: The present study was the first epidemiological study in Khorasan Razavi. This study allowed us to investigate the prevalence of Wolbachia bacteria in C. hemipterus in different populations from various areas of Mashhad city and compare it with the results of other similar studies.
Book Review: Review Article |
Subject:
Medical Parasitology
Received: 2024/03/9 | Accepted: 2024/04/9
Received: 2024/03/9 | Accepted: 2024/04/9
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