Volume 3, Issue 3 (11-2023)
Zoonosis 2023, 3(3): 57-65 |
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Babagolzadeh M, Taiefi N, Moghadas E, Moshaverinia A. Molecular characterization of Wolbachia infection in bedbugs (Cimex hemipterus) collected from Mashhad city, Iran. Zoonosis 2023; 3 (3) :57-65
URL: http://zoonosis.ir/article-1-85-en.html
URL: http://zoonosis.ir/article-1-85-en.html
Department of pathobiology, Islamic Azad University, Karaj branch, Karaj, Iran , ZARE396@YAHOO.COM
Abstract: (573 Views)
Wolbachia bacteria, identified in many species of Arthropoda, are intracellular organisms transmitted vertically from one generation to the next. Wolbachia plays a significant role in the pathogenesis of filarial diseases. Despite numerous studies on the prevalence of Wolbachia in Cimex lectularis populations, there have been no published reports on its presence in tropical bed bug (Cimex hemipterus) populations in Iran. The present study aimed to investigate the molecular characterization of Wolbachia infection in C. hemipterus collected from Mashhad, Iran. Specimens were obtained from four different districts of Mashhad (regions 2, 4, 8, and 11), specifically from old apartment buildings and workers' dormitories. In total, 100 tropical bed bug specimens were collected. Molecular identification of Wolbachia was carried out by targeting the Wolbachia surface protein (wsp) gene and comparing the results with previously registered Wolbachia sequences in GenBank. According to the findings, 13 specimens (13%) from two distinct colonies in regions 4 and 8 tested positive for Wolbachia infection. Sequencing results revealed that the amplified genes were 100% identical to the sequences registered in GenBank under accession numbers AB475131 (Australia) and DQ842459 (USA). One representative sequence from the 13 positive samples was submitted to GenBank with accession number PP501141. This study represents the first epidemiological report on Wolbachia infection in C. hemipterus in Khorasan Razavi Province. It provides new insights into the molecular characteristics of Wolbachia in tropical bed bug populations in Mashhad and allows for comparison with similar studies conducted globally.
Book Review: Original Article |
Subject:
Medical Parasitology
Received: 2024/03/9 | Accepted: 2024/04/9 | Published: 2025/07/24
Received: 2024/03/9 | Accepted: 2024/04/9 | Published: 2025/07/24
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