Volume 3, Issue 4 (12-2023)
Zoonosis 2023, 3(4): 26-36 |
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Ethics code: IR.IAU.SHK.REC.1400.034
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nezaratizade S, Khamesipour F, Hejazi S H. In vitro Evaluation of the Antileishmanial Activity of Dracocephalum kotschyi Essential Oil Against Leishmania major Amastigotes Compared with Standard Drugs. Zoonosis 2023; 3 (4) :26-36
URL: http://zoonosis.ir/article-1-98-en.html
URL: http://zoonosis.ir/article-1-98-en.html
Food and Drug Research Center, Iran Food and Drug Administration, Ministry of Health and Medical Education , faham.khamesipour@yahoo.com
Abstract: (58 Views)
Leishmaniasis, a zoonotic parasitic disease, is a significant public health concern. Current therapeutic approaches, primarily based on pentavalent antimonial compounds, are associated with notable limitations and adverse effects, underscoring the urgent need for alternative, effective treatments. Medicinal plants offer promising avenues for the development of novel therapeutic agents. This study aimed to evaluate the antileishmanial activity of the essential oil of Dracocephalum kotschyi against the amastigote form of Leishmania major and compare its efficacy to standard drugs. In this experimental in vitro study, promastigotes of the standard L. major strain were cultured in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS). Infected macrophages containing parasite amastigotes were exposed to varying concentrations of D. kotschyi essential oil, with Amphotericin B used as the positive control. After incubation, cytotoxicity and amastigote survival rates were assessed using the MTT colorimetric assay, and optical density (OD) values were measured with an ELISA reader. The 50% inhibitory concentration (IC50) was calculated for both the essential oil and the control drug. Results revealed that D. kotschyi essential oil exhibited a dose-dependent inhibitory effect on the growth of intracellular amastigotes (p < 0.05). The IC50 of the essential oil against amastigotes was 1136.53 µg/mL, while the 50% cytotoxic concentration (CC50) on macrophage cells was 193.60 µg/mL. These findings highlight the potential of D. kotschyi essential oil as an effective antileishmanial agent, capable of eliminating infected macrophages. Consequently, this plant-derived compound represents a promising candidate for further in vivo studies aimed at developing topical formulations for the treatment of cutaneous leishmaniasis.
Book Review: Original Article |
Subject:
Medical Parasitology
Received: 2025/09/29 | Accepted: 2025/12/4 | Published: 2025/12/26
Received: 2025/09/29 | Accepted: 2025/12/4 | Published: 2025/12/26
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