Volume 3, Issue 1 (5-2023)                   Zoonosis 2023, 3(1): 1-11 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bonyadian M, Karimi S. Serological and molecular investigation of brucellosis among high-risk people in Shahrekord city in 1400. Zoonosis 2023; 3 (1) :1-11
URL: http://zoonosis.ir/article-1-75-en.html
Department of Health and Food Quality Control, Faculty of Vet.Med, Shahrekord University, Shahrekord, Iran. , Boniadian@sku.ac.ir
Abstract:   (466 Views)
Brucellosis is one of the five common infectious diseases between humans and animals, caused by contamination with Brucella. Since farmers, students, veterinary medicine staff, and slaughterhouse personnel are considered to be at risk of contamination with this bacterium, it is necessary to estimate the prevalence of the disease in these individuals. This study was conducted on 300 people with high occupational risk (farmers, veterinary staff, veterinary students, slaughterhouse personnel) in Shahrekord City, Iran. Blood serum samples were evaluated using Rose Bengal, Wright, Combs Wright, 2ME, and PCR tests. People's profile was recorded by questionnaire. Data were analyzed using Sigma stat 4 statistical software by McNemart test. The results of the Rose Bengal test showed that out of 300 samples, 46 (15.3%) had a positive reaction, but in the Wright test, 24 (8%) showed a positive reaction with a titer of 1.80 or higher. Wright's test revealed that 12.5% of farmers, 10% of slaughterhouse workers, 5.7% of veterinary staff, and 2% of veterinary students had positive titers against Brucella. In the 2ME test, the rates of positive cases (1:40 and more) among farmers were 8.8%, slaughterhouse workers 7.5%, veterinary staff 5%, and veterinary students 2%. DNA extraction and PCR tests on serum samples showed that 7.5% of farmers, 6.3% of slaughterhouse workers, 5% of veterinary staff, and 2% of veterinary students had the Brucella bacterium genome in their blood serum. The statistical test results showed a significant agreement between the results of the 2ME and PCR tests (P<0.01). According to the results of the present study, the prevalence of brucellosis among farmers, slaughterhouse workers, and butchers was higher than among other groups at risk. There is a close agreement between the results of 2ME and PCR tests; therefore, the PCR can be used as a valid test to diagnose brucellosis.
 
Full-Text [PDF 626 kb]   (34 Downloads)    
Book Review: Original Article | Subject: Infectious Disease
Received: 2023/04/4 | Accepted: 2023/04/14 | Published: 2023/11/17

References
1. Cutler SJ, Whatmore AM, Commander NJ. Brucellosis - new aspects of an old disease. Journal of Applied Microbiology. 2005; 98: 1270-81. [DOI:10.1111/j.1365-2672.2005.02622.x] [PMID]
2. Moreno E, Cloeckaert A, Moriyon I. Brucella evolution and taxonomy. Veterinary Microbiology. 2002; 90: 209-27. [DOI:10.1016/S0378-1135(02)00210-9] [PMID]
3. Rezaee MA, Rashidi A, Motaharinia Y, Hossaini W, Rahmani MR. Seroprevalence study of brucellosis among high-risk groups in comparison with other people of the population in Sanandaj (West of Iran). African Journal of Microbiology Research. 2012; 6: 51-57. https://doi.org/10.5897/AJMR11.1095 [DOI:10.5897/AJMR11.1095 .[In Persian]]
4. Delvecchio VG, Kapatral V, Redkar RJ, Guy P, Cesar M, Tamara L, et al. The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proceedings of the National Academy of Sciences of the Unithed States of America. 2002; 99: 443-448. https://doi.org/10.1073/pnas.221575398 [DOI:10.1073/pnas.221575398.] [PMID] [PMCID]
5. Goldstein J, Hoffman T, Frasch C, Lizzio EF, Beining PR, Hochstein D, et al. Lipo polysaccharide (LPS) from Brucella abortus is less toxic thme that from Escherichia coli, suggesting possible use of B. abortus or LPS from B. abortus as a carrier in vaccines. Infection and Immunity. 1992; 60(4): 1385-1389. https://doi.org/10.1128/iai.60.4.1385-1389.1992 [DOI:10.1128/iai.60.4.1385-1389.1992.] [PMID] [PMCID]
6. Ocholi RA, Kwaga JK, Ajogi I, Virginie M, Amaia ZR, Ward B, et al. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Veterinary Microbiology. 2004; 103: 47-53. https://doi.org/10.1016/j.vetmic.2004.06.012 [DOI:10.1016/j.vetmic.2004.06.012.] [PMID]
7. Elzer PH, Rowe GE, Enright FM, James TD , Alexander JW. Balb/c mice infected with Brucella abortus express protracted polyclonal response of both andisotypes. Immunology Letter.1994; 42: 145-150. [DOI:10.1016/0165-2478(94)90078-7] [PMID]
8. Michaux S, Paillisson J, Carles M, Bourg G, Allardet-Servent A, Ramuz M. Presence of two independent chromosomes in the Brucella melitensis16M genome. Journal of Bacteriology. 1993; 175(3): 701-705. https://doi.org/10.1128/jb.175.3.701-705.1993 [DOI:10.1128/jb.175.3.701-705.1993.] [PMID] [PMCID]
9. Taravati V, Salarilak S, Sadeghkhalili F. Seroepidemiology study of brucellosis in the community of livestock farmers, butchers and slaughterhouse workers in Urmia. Journal of Urmia Medical Sciences University.1386; 1: 436-441. [In Persian]
10. Hosein HI, EL-Nahass S, Rouby SR, ElNesr KA. Detection of Brucella in Tissues and in Formalin-Fixed Paraffin Embedded (FFPE) Specimens Using PCR. Advances in Animal and Veterinary Sciences. 2018; 6(2): 55-62. https://doi.org/10.17582/journal.aavs/2018/6.2.55.62 [DOI:10.17582/journal.aavs/2018/6.2.55.62 [In Persian]]
11. Pizaro J, Stephane M, Robert G, Gisou G, Alberto SL, Ignacio LG, et al. Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes. Infection and.Immunity. 1998; 66(12): 5711-5724. https://doi.org/10.1128/IAI.66.12.5711-5724.1998 [DOI:10.1128/IAI.66.12.5711-5724.1998.] [PMID] [PMCID]
12. Ko J, Splitter GA. Molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. Clinical Microbiology Reviews. 2003; 16: 65-78. [DOI:10.1128/CMR.16.1.65-78.2003] [PMID] [PMCID]
13. Mostafavi E, Asmand M. Trend of brucellosis in Iran from 1991 to IRAN. Journal of Epidemiology. 2012; 8: 93-100. [In Persian]
14. Mukhtar F, Kokab F. Brucella serology in abattoir workers. Journal of Ayub Medical College Abbottabad. 2008; 20(3): 57-61.
15. Anisur R, Berkvens D, David F. Seroprevalence and risk factors for brucellosis in a high-risk group of individuals in Bangladesh. Foodborne Pathogen and Disease. 2012; 9: 190-197. [DOI:10.1089/fpd.2011.1029] [PMID]
16. Mishal J, Ben N, Levin Y, Sherf S, Jafari J, Embon E, et al. Brucellosis outbreak: analysis of risk factors and serologic screening. International Journal of Molecular Medicine. 1999; 4: 655-8. [DOI:10.3892/ijmm.4.6.655] [PMID]
17. Beheshti S, Rezaian GR, Azad F. Seroprevalence of brucellosis and risk factors related to high risk occupational groups in Kazeroon, south of Iran. International Journal of Occupational and Environmental Medicine. 2010; 1: 62-68.
18. Nikokar I, Hosseinpour M, Asmar M, Pirmohbatei S, Hakeimei F, Razavei MT. Seroprevalence of Brucellosis among high risk individuals in Guilan, Iran. Journal of Medical Sciences. 2011; 16: 1366-1371. [In Persian]
19. Halling SM, Peterson-Burch BD, Bricker BJ, Richard LZ, Zhang Q, Ling-Ling L, et al. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. Journal of Bacteriology. 2005; 187: 2715-26 https://doi.org/10.1128/JB.187.8.2715-2726.2005 [DOI:10.1128/JB.187.8.2715-2726.2005.] [PMID] [PMCID]
20. Morata P, Queipo MI, Reguera JM, García-Ordoñez MA, Pichardo C, Colmenero JD. Posttreatment follow-up of brucellosis by PCR assay. Journal of Clinical Microbiology. 1999; 37: 4163-4166. https://doi.org/10.1128/JCM.37.12.4163-4166.1999 [DOI:10.1128/JCM.37.12.4163-4166.1999.] [PMID] [PMCID]
21. Al-Eias YA, AL Mofada SM. Cogenital Brucellosis. The Journal of Pediatric Infectious Disease. 1992; 221: 5-11.
22. Karimi A, Alborzi A, Rasooli M, KadivarMR. Prevalence of antibody to Brucella species in butchers, slaughterers and others. Eastern Mediterranean Health Journal. 2003; 9: 178-181. https://doi.org/10.26719/2003.9.1-2.178 [DOI:10.26719/2003.9.1-2.178. [In Persian]] [PMID]
23. Khosravani AM, Afshoon E, Yazdanpanah B. Seroepidemiological Study of Brucellosis in High Risk Groups in Boyerahmad 1384. Armaghan Danesh. 1384; 11(4): 89-96. [In Persian]
24. Jabeen Sh. Detection of Brucella species by PCR from human blood. Journal of Medical Sciences. 2021; 24: 21-24.
25. Sangari FJ, Cayon AM, Seoane A, García-Lobo JM. Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system. Biomedical Central of Microbiology. 2010; 107: 1-12. [DOI:10.1186/1471-2180-10-107] [PMID] [PMCID]
26. Taghipour A, Sheikhaleslami N, Arsangjang S. Epidemiology of Malt fever and related factors in Qom province during 1380-90. Alborz University Medical Journal. 1391; 1(4): 193-199. [In Persian]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 All Rights Reserved | Journal of Zoonosis

Designed & Developed by : Yektaweb